HLA-E antibody

• Catalog Number: orb44423
• Category: Antibodies
• Description: Mouse Monoclonal to HLA-E.
• Clonality: Monoclonal
• Clone Number: MEM-E/07
• Tested applications: FC, IHC-P, IP
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide.
• Concentration: 1 mg/ml
• Dilution range: Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: spleen. Flow cytometry: Recommended dilution: 1-4 µg/ml
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: HLA-E
• Entrez: 3133
• UniProt ID: P13747
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-HLA-E antibody
• Note: For research use only
• Application notes: Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: spleen. Flow cytometry: Recommended dilution: 1-4 µg/ml
• Expiration Date: 12 months from date of receipt.

Separation of HLA-E transfected LCL cells stained using anti-human HLA-E (MEM-E/07) purified antibody (concentration in sample 2 µg/ml, red-filled) from HLA-E transfected LCL cells stained using mouse IgG1 isotype control (MOPC-21) purified antibody (concentration in sample 2 µg/ml, same as HLA-E purified antibody concentration, black-dashed) in flow cytometry analysis (surface staining).

Immunohistochemistry staining of human spleen (paraffin sections) with anti-HLA-E (clone MEM-E/07).