HLA-DRB (MHC II) Antibody

• Catalog Number: orb607423
• Category: Antibodies
• Description: Mouse monoclonal antibody to HLA-DRB (MHC II)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: DA2
• Tested applications: FC, IF, IHC, WB
• Reactivity: Human, Monkey
• Isotype: IgG2b
• Immunogen: Activated human peripheral blood mononuclear cells
• Concentration: Purified Ab with BSA and Azide at 200ug/ml
• Dilution range: Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Western Blot (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.
• Form/Appearance: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Conjugation: Unconjugated
• MW: ~28kDa (beta chain)
• Target: HLA-DRB1
• Entrez: 3123
• UniProt ID: P01911
• Expression System: Bioreactor
• Storage: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
• Buffer/Preservatives: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Alternative names: DRB1; HLA class II histocompatibility antigen, DR-
• Note: For research use only
• Application notes: Positive Control: Ramos, Daudi or HuT78 cells. Tonsil or lymph node.
• Expiration Date: 12 months from date of receipt.