HLA-DPB1, HLA-DQA1 Antibody

• Catalog Number: orb1252224
• Category: Antibodies
• Description: HLA-DPB1, HLA-DQA1 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CR3/43
• Tested applications: IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG1, kappa
• Immunogen: Human tonsil cells were used as the immunogen for the HLA-DP/DQ/DR antibody.
• Concentration: 0.2 mg/mL
• Dilution range: WB: 1-2 ug/mlImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RTOptimal dilution of the HLA-DP/DQ/DR antibody should be determined by the researcher.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: HLA-DPB1, HLA-DQA1
• UniProt ID: P01909, P01903, P01908, P04440, P01920
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Note: For research use only
• Application notes: WB: 1-2 ug/mlImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RTOptimal dilution of the HLA-DP/DQ/DR antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human tonsil tissue with HLA-DP/DQ/DR antibody (clone CR3/43). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.

IHC testing of FFPE human tonsil tissue with HLA-DP/DQ/DR antibody (clone CR3/43). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.

Western blot testing of human Raji cell lysate with HLA-DP/DQ/DR antibody (clone CR3/43). Expected molecular weight ~36 kDa (alpha chain) and ~27 kDa (beta chain).

SDS-PAGE Analysis of Purified, BSA-Free HLA-DP/DQ/DR Antibody (clone CR3/43). Confirmation of Integrity and Purity of the Antibody.

IF/ICC staining of human Raji cells with HLA-DP/DQ/DR antibody (green) and Reddot nuclear stain (red).

Flow cytometry testing of human Raji cells with HLA-DP/DQ/DR antibody at 1 ug/10^6 cells (blocked with goat sera); Red = isotype control, Blue = HLA-DP/DQ/DR antibody.