HLA-DOB antibody

• Catalog Number: orb214037
• Category: Antibodies
• Description: Rabbit polyclonal antibody to HLA-DOB
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human HLA-DOB. The exact sequence is proprietary.
• Dilution range: WB: 1-500-1-1000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: HLA-DOB
• Entrez: 3112
• UniProt ID: P13765
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti HLA class II histocompatibility antigen, DO b
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of HLA-DOB expression in mouse lung (A), rat lung (B) whole cell lysates. (Predicted band size: 30 kD; Observed band size: 31 kD)

Immunohistochemical analysis of HLA-DOB staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.