HLA-B7 antibody (PE)

• Catalog Number: orb402821
• Category: Antibodies
• Description: Mouse monoclonal antibody against HLA-B7 conjugated to PE
• Clonality: Monoclonal
• Clone Number: BB7.1
• Tested applications: FC
• Reactivity: Human, Primate
• Isotype: Mouse IgG1
• Immunogen: Papain solubilised HLA-A2, B7
• Dilution range: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Purity: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: PE
• Target: HLA-B7
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Note: For research use only
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Expiration Date: 12 months from date of receipt.

Separation of human lymphocytes of HLA-B7 positive blood donor (red-filled) from human lymphocytes of HLA-B7 negative blood donor (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood samples stained using anti-HLA-B7 (BB7.1) PE antibody (10 µl reagent / 100 µl of peripheral whole blood).

Flow cytometry surface staining patterns of human peripheral whole blood of HLA-B7 positive (left) and negative (right) blood donors stained using anti-HLA-B7 (BB7.1) PE antibody (10 µl reagent / 100 µl of peripheral whole blood).