HLA-A+B+C+E antibody

• Catalog Number: orb421959
• Category: Antibodies
• Description: Mouse monoclonal antibody to HLA-A+B+C+E
• Clonality: Monoclonal
• Clone Number: TP25.99SF
• Tested applications: FC, IHC-Fr, WB
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: IFN-gamma-treated human melanoma cells Colo 38
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 1-4 μg/ml. Western blotting: Recommended dilution: 1-2 µg/ml; non-reducing conditions.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: HLA-ABCE
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1-4 μg/ml.Western blotting: Recommended dilution: 1-2 µg/ml; non-reducing conditions.
• Expiration Date: 12 months from date of receipt.

Flow cytometry analysis (surface staining) of human peripheral blood cells using anti-HLA-ABCE (TP25.99SF) purified, GAM-APC.

Anti-HLA-ABCE Purified (clone TP25.99SF) works in WB application. Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of JAR, JEG3, Raji, and K562 cell lines, mixed and heated (100°C, 5 min) with non-reducing SDS-loading buffer. Samples were resolved using 10% Tris-glycine SDS gel electrophoresis. Nitrocellulose membrane blot was probed with mouse IgG1 monoclonal antibody TP25.99SF (2 µg/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green). Mouse IgG1 anti-GAPDH monoclonal antibody FF26A conjugated with DyLight 680 (1 µg/ml), was used as the loading control (red). Multiplex fluorescent Western blot detection was performed. HLA-ABCE molecules were detected at ~40 kDa in JAR and Raji cell lines.