HLA-A2 Antibody

• Catalog Number: orb1906406
• Category: Antibodies
• Description: HLA-A2 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: BB7.2
• Tested applications: ELISA, FC, IF, IHC, WB
• Reactivity: Human
• Isotype: IgG2b
• Immunogen: The original antibody was generated by immunizing BALB/c mice with papain solubilized HLA-A2 and B7 antigens.
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• Target: HLA-A2
• UniProt ID: P04439
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: HLA-A; Human leukocyte antigen A; HLA class I hist
• Note: For research use only
• Application notes: The binding specificity of BB7.2 towards HLA-A2 was assessed using complement-mediated cytotoxicity and cellular radio binding assay. This antibody is cytotoxic for peripheral blood leukocytes and lymphoid cell lines. The original IgG2b version of this antibody binds HLA-A2 positive cells with a binding or avidity constant of about 5 x 108 liter/mol (PMID:7035415). This antibody was used to detect HLA-A*0201-PR1 complexes using western blot and ELISA (PMID: 17493346). This antibody was also used to detect HLA-A2 complexed with pol, gag, and EBV peptides using ELISA (PMID:8562505). This antibody was used to screen human leucocyte antigen (HLA)-A2 haplotype-positive HBV patients using flow cytometry (PMID: 16108766). This antibody was also used for the immunofluorescent staining of HLA-A2.1 molecules on the cell surface of human processing defective cell line (T2) (PMID:7684681). This antibody was used in a study involving the expression of HLA class molecules in head and neck squamous cell carcinoma (HNSCC) specimens by immunohistochemistry (PMID: 9328140).
• Expiration Date: 12 months from date of receipt.