Histone H4 (phospho-S47) antibody

• Catalog Number: orb224030
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Histone H4 (Phospho-S47)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Rat
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S47 of human Histone H4 protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1:1000, IF/ICC: 1:100-1:500
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: HIST1H4A
• Entrez: 8361, 121504, 8367, 8365, 8359, 8364, 8366, 8360, 8362, 8370, 8368, 8294, 8363, 554313
• UniProt ID: P62805
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti H4/A antibody, anti H4FA antibody, anti H4/I
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Histone H4 (Phospho-S47) expression in HEK293T (A), U2OS (B) whole cell lysates. (Predicted band size: 11 kD; Observed band size: 11 kD)

Immunofluorescent analysis of Histone H4 (Phospho-S47) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.