Histone H4 (MonoMethyl-R3) antibody

• Catalog Number: orb1289771
• Category: Antibodies
• Description: Rabbit Polyclonal antibody to Histone H4
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human, Mouse, Porcine, Rat
• Immunogen: KLH-conjugated synthetic Mono-methylated peptide corresponding to residues surrounding R3 of human Histone H4 protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1:1000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; HIST2H4A; HIST2H4B; HIST4H4
• Entrez: 8361, 319159, 102557184, 100041230, 319158, 8360, 69386, 502913, 8370, 8368, 8294, 102548682, 319156, 100360950, 121504, 319161, 8359, 8366, 500351, 319157, 295277, 320332, 319155, 8367, 8365, 8364, 291152, 100912290, 102641229, 8363, 554313, 102551184, 326619, 319160, 680097, 97122, 100912564, 64627, 100912418, 8362, 326620
• UniProt ID: P62806, P62804, P62805
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: H4/A; H4FA; H4/I; H4FI; H4/G; H4FG; H4/B; H4FB; H4
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Histone H4 (MonoMethyl-R3) expression in HEK293T (A), A549 (B), U2OS (C) whole cell lysates. (Predicted band size: 11 kD; Observed band size: 14 kD)

Direct ELISA antibody dose-response curve using Anti-Histone H4 (MonoMethyl-R3) Antibody. Antigen (methyl-peptide and non-methyl-peptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.