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Catalog Number | orb420436 |
---|---|
Category | Antibodies |
Description | Histone H4 K12-Ac antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | DOT, IF, IHC, Multiplex Assay, WB |
Reactivity | Human |
Isotype | IgG |
Immunogen | Histone H4 [ac Lys12] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic acetylated peptide surrounding Lysine 12 of human Histone H4. |
Concentration | 0.69 mg/mL |
Dilution range | IHC: 1:100, IF: 1:10, WB: 1.0 ug/mL |
Form/Appearance | Liquid (sterile filtered) |
Purity | Anti-Histone H4 [ac Lys12] was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with Alfalfa based on 100% sequence homology. Cross-reactivity with Histone H4 [ac Lys12] from other sources has not been determined. |
Conjugation | Unconjugated |
UniProt ID | P62805 |
NCBI | NP_001029249 |
Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | rabbit anti-Histone H4 Ac Lys12 antibody, H4K12ac, Read more... |
Note | For research use only |
Application notes | Anti-Histone H4 K12 ac antibody is tested for Western Blot, Dot Blot, and Immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~11 kDa corresponding to Histone H4 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus antibody production in collaboration with Novus Biologicals. |
Expiration Date | 12 months from date of receipt. |
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