Histone H3 (TriMethyl K9) antibody

• Catalog Number: orb323167
• Category: Antibodies
• Description: Mouse monoclonal antibody to Histone H3 (TriMethyl-K9)
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: IH, IP, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic Tri-methylated peptide corresponding to residues surrounding K9 of human Histone H3 protein. The exact sequence is proprietary.
• Dilution range: WB: 1:1000-1:3000, IHC-P: 1:200-1:500, IP: 1:100-1:200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: HIST1H3A; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST2H3A; HIST2H3C; HIST2H3D; H3F3A; H3F3B
• Entrez: 8352, 8356, 8354, 8968, 15078, 8357, 319152, 8358, 8355, 291159, 100361558, 8350, 15077, 8353, 8351
• UniProt ID: P68431, P68433, Q6LED0, P84244, P84228, Q71DI3, P84245, P84243
• Source: Mouse
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti HIST1H3A antibody, anti H3FA antibody, anti H
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Histone H3 (TriMethyl-K9) expression in Hela (A) whole cell lysates. (Predicted band size: 15 kD; Observed band size: 17 kD)

Immunohistochemical analysis of Histone H3 (TriMethyl-K9) staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunoprecipitation of Histone H3 (TriMethyl-K9) from 0.5 mg Hela whole cell extract lysate, using Anti-Histone H3 (TriMethyl-K9) Antibody.