Histone H3 pT11 antibody

• Catalog Number: orb420403
• Category: Antibodies
• Description: Histone H3 pT11 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ChIP, DOT, IF, IHC, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Histone H3 [p Thr11] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic phosphorylated peptide surrounding Threonine 11 of human Histone H3.2.
• Concentration: 1.2 mg/ml
• Dilution range: ChIP: 2-5µg/million cells, IHC: 1:50, IF: 1:50, WB: 1:1000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-Histone H3 [p Thr11] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H3.2. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: Q71DI3
• NCBI: NP_001005464
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Histone H3 pT11 antibody, H3.3B, H3.3A
• Note: For research use only
• Application notes: Anti-Histone H3 [p Thr11] antibody is tested for Western Blot, Dot Blot, and Immunofluorescence. This antibody is useful for Chromatin Immunoprecipitation and Immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus antibody production in collaboration with Novus Biologicals.
• Expiration Date: 12 months from date of receipt.