Histone H3, Phospho (S10) Antibody

• Catalog Number: orb1527687
• Category: Antibodies
• Description: Histone H3, Phospho (S10) Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IHC, WB
• Reactivity: Human, Mouse
• Immunogen: surrounding serine 10
• Concentration: 200 µg/ml
• Dilution range: WB - 1:5,000 - 1:10,000; IHC - 1:2,000 - 1:10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.; ICC-IF - 1:2,000 - 1:10,000. Formaldehyde fixation and permeabilization with Triton-X 100 is recommended. Acetone fixation may adversely effect staining.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: Histone H3, Phospho (S10)
• UniProt ID: P68431
• NCBI: NP_003520.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: Histone H3/c; Histone H3/k; Histone H3/l; Histone
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human Phospho Histone H3 (pS10) by western blot. A-549, HeLa, K-562, and Jurkat cell lines were stimulated with 0.5 µg/ml of Calyculin A at 37°C, 5% CO₂ for 15 minutes. Cells were subsequently lysed and 1 µg of appropriate cell lysates were loaded into respective wells. Western blot was performed using affinity purified rabbit anti-Histone H3 (pS10) antibody orb1527687 (20 ng/ml) and affinity purified rabbit anti-Histone H3 antibody (1 µg/ml). Western blots were developed utilizing chemiluminescence with an exposure time of 30 seconds.

Detection of human and mouse Histone H3 (S10) by immunohistochemistry.Sample: FFPE section of human colon carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-Phospho Histone H3 (S10) (Cat. No. orb1527687) used at a dilution of 1: 2, 500 (0.08 µg/ml). Detection: DAB

Detection of human Phospho Histone H3 (S10) by immunocytochemistry. Samples: NBF-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-Phospho Histone H3 (S10) (Cat. No. orb1527687) used at a dilution of 1: 2, 500 (0.08µg/ml). Detection: Green fluorescent goat antirabbit IgG-FITC conjugated used at a dilution of 1: 100.