Histone H3 (MonoMethyl K26) antibody

• Catalog Number: orb1289778
• Category: Antibodies
• Description: Rabbit Polyclonal antibody to Histone H3
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Rat, Zebrafish
• Immunogen: KLH-conjugated synthetic Mono-methylated peptide corresponding to residues surrounding K27 of human Histone H3 protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1:1000, IH: 1:50-1:200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: HIST1H3A; HIST1H3; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST2H3A; HIST2H3C; HIST2H3D; H3F3A; H3F3B; H3F3C
• Entrez: 8352, 8356, 8354, 8968, 8357, 126961, 3021, 8358, 8355, 3020, 8350, 440093, 8353, 333932, 653604, 8351
• UniProt ID: Q6NXT2, P68431, Q71DI3, P84243
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HI
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Histone H3 (MonoMethyl-K27) expression in A549 (A), H1688 (B) whole cell lysates. (Predicted band size: 15 kD; Observed band size: 17 kD)

Immunohistochemical analysis of Histone H3 (MonoMethyl-K27) staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Direct ELISA antibody dose-response curve using Anti-Histone H3 (MonoMethyl-K27) Antibody. Antigen (methyl-peptide and non-methyl-peptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.