Histone H3 antibody

• Catalog Number: orb339084
• Category: Antibodies
• Description: Rabbit polyclonal antibody to HIST1H3A
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human, Mouse, Porcine, Rat, Zebrafish
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Histone H3. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: HIST1H3A; HIST1H3; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST2H3A; HIST2H3C; HIST2H3D; H3F3A; H3F3B; H3F3C
• Entrez: 8352, 8356, 8354, 8968, 8357, 126961, 3021, 8358, 8355, 3020, 8350, 440093, 8353, 333932, 653604, 8351
• UniProt ID: Q6NXT2, P68431, Q71DI3, P84243
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti HIST1H3A antibody, anti H3FA antibody, anti H
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Histone H3 expression in NIH3T3 (A) whole cell lysates. (Predicted band size: 15 kD; Observed band size: 17 kD)

Direct ELISA antibody dose-response curve using Anti-Histone H3 (DiMethyl-K9) Antibody. Antigen (methyl-peptide and non-methyl-peptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.