Histone H3 Antibody

• Catalog Number: orb1529569
• Category: Antibodies
• Description: Histone H3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: between 100 and C-term
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:200 - 1:1,000. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.; IHC-IF - 1:100 - 1:500
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: Histone H3
• UniProt ID: P68431
• NCBI: NP_003520.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: Histone H3/c; Histone H3/k; Histone H3/l; Histone
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of mouse Histone-H3 by western blot.Samples: Whole cell lysate from TCMK-1 and NIH 3T3 cells prepared using RIPA lysis buffer. Antibody: Affinity purified rabbit anti-Histone-H3 antibody orb1529569 at 0.1 µg/ml.

Detection of human Histone H3 by western blot.Samples: Histones isolated from HeLa cells. Antibody: Affinity purified rabbit anti-Histone H3 antibody orb1529569 at 0.1 µg/ml.

Detection of human Histone H3 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Histone H3 used at a dilution of 1:1, 000 (0.2µg/ml).

Detection of human Histone H3 by western blot of immunoprecipitates.Samples: Histones isolated from HeLa cells. Antibodies: Affinity purified rabbit anti-Histone H3 antibody.

IHC: Sample: human prostate carcinoma. Antibody: Affinity purified rabbit anti-Histone H3 used at a dilution of 1:200. Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain, cross-adsorbed Antibody DyLight® 594 Conjugated.