Histone H3 antibody

• Catalog Number: orb136531
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Histone H3
• Clonality: Polyclonal
• Tested applications: ChIP, IHC, IP, WB
• Reactivity: All, Human, Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic peptide of human HIST3H3(NP_003484.1).
• Concentration: 1 mg/ml
• Dilution range: WB: 1:500-2000, IHC-P: 1:50-200, IF/ICC: 1:50-200, IP: 1:50-200, ChIP: 1:50-200
• Conjugation: Unconjugated
• MW: 17KDa
• UniProt ID: Q16695
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.05% proclin300,50% glycerol,pH7.3.
• Alternative names: anti HIST1H3J antibody, anti H3/j antibody, anti H
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Zheng, Zu-Guo et al. Anhydroicaritin improves diet-induced obesity and hyperlipidemia and alleviates insulin resistance by suppressing SREBPs activation Biochem Pharmacol, 122, 42-61 (2016)

PubMed: 27816546

Sun, Jun et al. Histone demethylase LSD1 regulates bone mass by controlling WNT7B and BMP2 signaling in osteoblasts Bone Res, 6, 14 (2018)

PubMed: 29707403

IHC-P image of human lung cancer tissue using Histone3 antibody (primary antibody dilution at 1:200)

IHC-P staining of human liver cancer tissue using Histone3 antibody (primary antibody dilution at 1:200)

Immunohistochemical analysis of formalin-fixed and paraffin-embedded human rectal cancer tissue using Histone3 antibody (primary antibody dilution at 1:200)

Chromatin Immunoprecipitation analysis extracts of HeLa cell line Histone H3 antibody

Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli using Histone3 antibody

Immunohistochemical staining of paraffin embedded rat kidney tissue using Histone3 antibody (primary antibody dilution at 1:200)