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    Histone H3 (AcK27) antibody

    Catalog Number: orb1474466

    DispatchUsually dispatched within 5-10 working days
    $ 489.00
    Catalog Numberorb1474466
    CategoryAntibodies
    DescriptionRabbit monoclonal antibody to Histone H3 (Acetyl-K27)
    Species/HostRabbit
    ClonalityMonoclonal
    Tested applicationsChIP, IF, IH, IP, WB
    ReactivityHuman
    ImmunogenKLH-conjugated synthetic acetylated peptide corresponding to residues surrounding K27 of human Histone H3 protein. The exact sequence is proprietary.
    Dilution rangeWB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/50 - 1/100), IP (1/10 - 1/50), ChIP (1/10 - 1/50)
    Form/AppearanceLiquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
    ConjugationUnconjugated
    TargetH3C1
    Entrez8350
    UniProt IDP68431
    SourceRabbit
    StorageShipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
    Buffer/PreservativesLiquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
    Alternative namesH3FA; H3FL; H3FC; H3FB; H3FD; H3FI; H3FH; H3FK; H3
    Read more...
    NoteFor research use only
    Expiration Date12 months from date of receipt.
    Histone H3 (AcK27) antibody

    Western blot analysis of Histone H3 (Acetyl-K27) expression in Hela (A), A549 (B), HL60 (C) whole cell lysates. (Predicted band size: 15 kD; Observed band size: 15 kD)

    Histone H3 (AcK27) antibody

    Immunohistochemical analysis of Histone H3 (Acetyl-K27) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.91). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    Histone H3 (AcK27) antibody

    Immunofluorescent analysis of Histone H3 (Acetyl-K27) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.

    • Histone H3 (AcK27) antibody [orb256598]

      IF,  IH,  WB

      Human, Mouse, Porcine, Primate, Rabbit, Rat, Zebrafish

      Rabbit

      Polyclonal

      Unconjugated

      200 μl, 100 μl, 30 μl
    • Histone H3 (AcK27) antibody [orb334689]

      IH,  WB

      Human, Mouse, Rat

      Rabbit

      Polyclonal

      Unconjugated

      200 μl, 100 μl, 30 μl
    • Histone H3 (AcK27) peptide [orb256888]

      Blocking

      > 85%

      Synthetic

      5 mg, 1 mg
    • Histone H3 (AcK27) antibody [orb1187976]

      IF,  IHC,  WB

      Bovine, Gallus, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Zebrafish

      Rabbit

      Polyclonal

      Unconjugated

      50 μl, 100 μl
    • Histone H3 (AcK27) antibody [orb1190001]

      IP,  WB

      Human, Mouse, Rat

      Rabbit

      Polyclonal

      Unconjugated

      100 μl, 50 μl
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