Histone H3 (AcK23) antibody

• Catalog Number: orb256597
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Histone H3 (Acetyl-K23)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Porcine, Rabbit, Rat, Zebrafish
• Immunogen: KLH-conjugated synthetic acetylated peptide corresponding to residues surrounding K23 of human Histone H3 protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1000, IF/ICC: 1:100-500
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: HIST1H3A; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST2H3A; HIST2H3C; HIST2H3D; H3F3A; H3F3B
• Entrez: 8352, 319152, 291159, 100361558, 8354, 8358, 3020, 8350, 15077, 8353, 333932, 653604, 15078, 126961, 3021, 8355, 8351, 8356, 8968, 8357
• UniProt ID: P68431, P68433, Q6LED0, P84244, P84228, Q71DI3, P84245, P84243
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti HIST1H3A antibody, anti H3FA antibody, anti H
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Histone H3 (Acetyl-K23) expression in HEK293T (A), Hela (B) whole cell lysates. (Predicted band size: 15 kD; Observed band size: 17 kD)

Immunofluorescent analysis of Histone H3 (Acetyl-K23) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).