Histone H2B (Butyryl-K5) antibody

• Catalog Number: orb1289923
• Category: Antibodies
• Description: Rabbit Polyclonal antibody to Histone H2B
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Rat, Zebrafish
• Immunogen: KLH-conjugated synthetic butylated peptide corresponding to residues surrounding K5 of human Histone H2B protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1:1000, IH: 1:50-1:200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: HIST1H2BC
• Entrez: 8344, 8346, 24829, 319178, 319179, 8339, 255626, 8347, 319177, 3017, 3018, 8343
• UniProt ID: P62807, Q6ZWY9, P70696, P33778, Q00729, Q64475
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: HIST1H2BA; TSH2B; Histone H2B type 1-A; Histone H2
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Histone H2B (Butyryl-K5) expression in HEK293T (A), U2OS (B), DLD (C) whole cell lysates. (Predicted band size: 13 kD; Observed band size: 14 kD)

Immunohistochemical analysis of Histone H2B (Butyryl-K5) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Direct ELISA antibody dose-response curve using Anti-Histone H2B (Butyryl-K5) Antibody. Antigen (butyryl-peptide and non-butyryl-peptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.