Histone H2B (AcK125) antibody

• Catalog Number: orb1289784
• Category: Antibodies
• Description: Rabbit Polyclonal antibody to Histone H2B
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human, Mouse, Rat, Zebrafish
• Immunogen: KLH-conjugated synthetic acetylated peptide corresponding to residues surrounding K125 of human Histone H2B protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1:1000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: HIST1H2BC
• Entrez: 8344, 8346, 24829, 319178, 319179, 8339, 255626, 8347, 319177, 3017, 3018, 8343
• UniProt ID: P62807, Q96A08, P70696, Q6ZWY9, P33778, Q00729, Q64475
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: HIST1H2BA; TSH2B; Histone H2B type 1-A; Histone H2
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Histone H2B (Acetyl-K125) expression in SP20 (A), SHSY5Y (B), C6 (C), PC3 (D) whole cell lysates. (Predicted band size: 14; 13 kD; Observed band size: 17 kD)

Direct ELISA antibody dose-response curve using Anti-Histone H2B (Acetyl-K125) Antibody. Antigen (Acetyl-peptide and non-acetyl-peptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.