His tag Mouse Monoclonal Antibody

• Catalog Number: orb500509
• Category: Antibodies
• Description: His tag Mouse Monoclonal Antibody
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: All
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein G
• Immunogen: KLH conjugated HHHHHH peptide
• Tested applications: ELISA, WB
• Dilution range: WB=1:5000-50000, ELISA=1:1000-5000
• Antibody Type: Primary Antibody
• Clone Number: 6E6
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: HisX6; 6His; His-Tag; 6 His epitope tag; Hexa His
• Research Area: 6x his tag, His Tag
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: His-Tagged Fusion Protein Overexpression E.coli Lysate, Primary: Anti-His tag (orb500509) at 1/200000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 41 kDa, Observed band size: 45 kDa.

Sample: Lane 1: His-Tagged Fusion Protein Overexpression E.coli Lysate (orb1516792) at 2 ug Lane 2: His-Tagged Fusion Protein Overexpression E.coli Lysate (orb1516792) at 2 ug Primary: Anti-His tag (orb500509) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 45 kD.