His Tag Antibody

• Catalog Number: orb1927575
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB19960
• Tested applications: WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:8000, WB: 1:1000, WB: 1:1000
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Target: Poly-HIS peptide were used to produced this antibody.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Poly-His, Hexa-HIS, Hexa-Histidine
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of His Tag Antibody in his-tag TBB1 protein (35 ug/lane). His-tag TBB1 (arrow) was detected using the purified Pab.

Anti-His Tag at 1:8000 dilution + 12tag recombination protein. Lysates/proteins at 20 ng per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 42 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-His Tag Antibody at 1:1000 dilution. Lane 1: 293T/17 whole cell lysate. Lane 2: 293T/17 transfected with 12tag whole cell lysate. Lane 3: 12tag recombinant protein. Lysates/proteins at 1µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 45 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.