HIPK2 Rabbit Polyclonal Antibody

• Catalog Number: orb100602
• Category: Antibodies
• Description: HIPK2 Rabbit Polyclonal Antibody
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Canine, Gallus, Porcine, Rabbit
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated synthetic peptide derived from human HIPK2 (401-500/1198aa)
• UniProt ID: Q9H2X6
• MW: 131 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: hHIPk 2; hHIPk2; HIPK 2; Hipk2; HIPK2_HUMAN; Homeo
• Note: For research use only

Sample: Muscle (Mouse) Lysate at 40 ug, Kidney (Mouse) Lysate at 40 ug, Primary: Anti-HIPK2 (orb100602) at 1/300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1/5000 dilution, Predicted band size: 131 kD, Observed band size: 132 kD.

Tissue/cell: human kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-HIPK2 Polyclonal Antibody, Unconjugated (orb100602) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.