HighYield T73M Aptamer Synthesis Kit (2'F-dUTP)

• Catalog Number: orb692287
• Category: Tools
• Description: HighYield T73M Aptamer Synthesis Kit (2'F-dUTP) is designed to produce large amounts of 2'-Fluoro-modified RNA via in vitro transcription with T73M RNA polymerase. 2'-fluoro modified RNA exhibits an increased RNA stability and is ideally suited for aptamer preparation. A modified T7 RNA poly-merase (T73M RNA polymerase) ensures the efficient incorporation of 2'-fluoro modified dUTP instead of is natural counterpart UTP. The kit contains sufficient reagents for 30 reactions á 20 µl (7.5 mM ATP, 7.5 mM CTP, 7.5 mM GTP, 7.5 mM 2'-Fluoro-dUTP). An individual optimization of 2'-Fluoro-dUTP concentration can easily be achieved with the single nucleotide format. A 20 µl reaction yields about 20 µg RNA after 30 min incuba-tion (1 µg T7 control template, 1.4 kb RNA transcript). Yields may however vary depending on the template (promotor design, sequence length, secondary structure formation).
• Hazard Information: Non-Toxic
• Storage: store at -20 °C
• Note: For research use only
• Expiration Date: 12 months from date of receipt.