HighYield T7 Cy5 RNA Labeling Kit (UTP-based)

SKU: orb611240

Description

HighYield T7 Cy5 RNA Labeling Kit (UTP-based) is designed to produce randomly Cy5-modified RNA probes via in vitro transcription. Such probes are ideally suited for in situ hybridization and Northern Blot experiments. UTP-X-CY5 (also known as Cy5-17-UTP from GE Healthcare) is effi-ciently incorporated into RNA as substitute for its natural counterpart UTP using an optimized reaction buffer and T7 RNA Labeling Poly-merase Mix. 35% UTP-X-Cy5 substitution typically results in an optimal balance between reaction and labeling efficiency. Individual optimization of UTP-X-Cy5/UTP ratio however, can easily be achieved with the single nucleotide format. The resulting Cy5-modified RNA probe can subsequently be detected by fluorescence spectroscopy. The kit contains sufficient reagents for 20 labeling reactions of 20 µl each (35% UTP-X-Cy5 substitution, 2.5 mM ATP, GTP, CTP, 0.2 mM UTP, 0.1 mM UTP-X-Cy5).

Images & Validation

• Application Notes:
  Spectroscopic Propertie: λexc 649 nm, λem 670 nm, ε 250.0 L mmol-1, cm-1 (Tris-HCl pH 7.5).

Key Properties

• SMILES: C(/C=C/c1cn(c(=O)[nH]c1=O)[C@H]1[C@@H]([C@@H]([C@H](O1)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)NC(=O)CCCCCNC(=O)CCCCCN1/C(=C/C=C/C=C/C2=[N+](CC)c3c(C2(C)C)cc(S(=O)(=O)[O-])cc3)/C(c2c1ccc(c2)S(=O)(=O)O)(C)C

Storage & Handling

• Storage: store at -20 °C
• Expiration Date: 12 months from date of receipt.
• Hazard Information: Non-Toxic
• Disclaimer: For research use only

Alternative Names

Preparation of randomly Cy5-modified RNA probes by in vitro transcription with UTP-X-CY5

Compound Identifiers

PubChem CID

176417997