HighYield T7 AF555 RNA Labeling Kit

• Catalog Number: orb1176568
• Category: Tools
• Description: HighYield T7 AF555 RNA Labeling Kit is designed to produce randomly AF555-modified RNA probes via in vitro transcription. Such probes are ideally suited for in situ hybridization and Northern Blot experiments. AF55 (a structural analog of Alexa Fluor 555) is a hydrophilic dye with increased photostability compared to Cy3. UTP-PEG5-AF555 is efficiently incorporated into RNA as substi-tute for its natural counterpart UTP using an optimized reaction buffer and T7 RNA Labeling Polymerase Mix. 35% UTP-PEG5-AF555 substitution typically results in an optimal balance between reaction and labeling efficiency. Individual optimization of UTP-PEG5-AF555/UTP ratio however, can easily be achieved with the single nucleotide format. The resulting AF555-modified RNA probe can subsequently be detected by fluorescence spectroscopy. The kit contains sufficient reagents for 20 labeling reactions of 20 µl each (35% UTP-PEG5-AF555 substitution, 2.5 mM ATP, GTP, CTP, 0.2 mM UTP, 0.1 mM UTP-PEG5-AF555). A 20 µl reaction yields about 5-7 µg RNA after 30 min incuba-tion (1 µg T7 control template, 1.4 kb RNA transcript). Yields may however vary depending on the template (promotor design, sequence length, secondary structure formation).
• SMILES: P(=O)(OP(=O)(O)O)(O)OP(=O)(O)OC[C@H]1O[C@H]([C@@H]([C@@H]1O)O)n1c(=O)[nH]c(=O)c(c1)/C=C/CNC(=O)CCOCCOCCOCCOCCOCCNC(=O)CCCCC[N+]1=C(/C=C/C=C/2\C(c3c(N2CCCS(=O)(=O)O)ccc(c3)S(=O)(=O)O)(C)C)C(c2c1ccc(c2)S(=O)(=O)[O-])(C)CCCCS(=O)(=O)O
• Hazard Information: Non-Toxic
• Storage: store at -20 °C
• Note: For research use only
• Application notes: Spectroscopic Propertie: λexc 555 nm, λem 572 nm, ε 155.0 L mmol-1, cm-1 (Tris-HCl pH 7.5).
• Expiration Date: 12 months from date of receipt.