HIF3/HIF3A Antibody

• Catalog Number: orb614119
• Category: Antibodies
• Description: HIF3/HIF3A Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human HIF3/HIF3A recombinant protein (Position: Q29-Q480).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat Flow Cytometry, 1-3μg/1x106 cells, Human Direct ELISA, 0.1-0.5μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 72 kDa
• UniProt ID: Q9Y2N7
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: "Hypoxia-inducible factor 3-alpha; HIF-3-alpha; HI
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis using anti-HIF3A antibody.Lane 1:rat lung tissue, Lane 2:rat skeletal muscle tissue, Lane 3:mouse lung tissue, Lane 4:mouse NIH3T3 cell.

WB analysis using anti-HIF3A antibody.Lane 1:HeLa cell, Lane 2:A431 cell, Lane 3:Caco-2 cell, Lane 4:U-87MG cell, Lane 5:U2OS cell, Lane 6:K562 cell, Lane 7:PC-3 cell.

Flow Cytometry analysis of U87 cells using anti-HIF3A antibody(Blue line).Isotype control antibody (Green line) was rabbit IgG.Unlabelled sample (Red line) was also used as a control.