HIF1 beta/ARNT Antibody

• Catalog Number: orb215884
• Category: Antibodies
• Description: HIF1 beta/ARNT Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human HIF1 beta recombinant protein (Position: V416-E789). Human HIF1 beta shares 86% and 83% amino acid (aa) sequences identity with mouse and rat HIF1 beta, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 86636 MW
• UniProt ID: P27540
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Aryl hydrocarbon receptor nuclear translocator;ARN
• Note: For research use only
• Application notes: WB: The detection limit for HIF1 beta is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of HIF1 using anti-HIF1 antibody.Lane 1:recombinant human HIF1 beta protein 0.5 ng.

WB analysis of HIF1 using anti-HIF1 antibody.Lane 1:human HeLa cell; 2:human 293T cell; 3:human Jurkat cell; 4:human U87 cell; 5:human COLO320 cell.