HIF1 beta antibody

• Catalog Number: orb334654
• Category: Antibodies
• Description: Rabbit polyclonal antibody to HIF1 beta
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant protein corresponding to human HIF1 beta.
• Dilution range: WB: 1:1000-1:2000, IHC-P: 1:200-1:500
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: ARNT
• Entrez: 405
• UniProt ID: P27540
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti HIST1H1A antibody, anti H1F1 antibody, anti H
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of HIF1 beta expression in MCF7 (A), NIH3T3 (B), rat brain (C) whole cell lysates. (Predicted band size: 86 kD; Observed band size: 87 kD)

Immunohistochemical analysis of HIF1 beta staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.