HIF Prolyl Hydroxylases Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb2561541
• Category: Antibodies
• Description: HIF Prolyl Hydroxylases Recombinant Rabbit Monoclonal Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Tested applications: FC, IF, IHC-Fr, IHC-P, IP, WB
• Predicted Reactivity: Human
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human HIF PH4
• Antibody Type: Recombinant Antibody
• Dilution range: WB=1:1000-1:2000, IHC-P=1:100-1:200, IHC-F=1:100-1:200, IP=1:20-1:50, Flow-Cyt=1:20-1:100, IF=1:100-1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 47 kDa
• Target: P4HTM
• UniProt ID: Q9NXG6
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% rAlbumin, 0.02% sodium azide and 50% glycerol.
• Alternative names: EGLN4; HIFPH4; Hypoxia inducible factor prolyl 4 h
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of HeLa cell lysate. Using HIF Prolyl Hydroxylases (orb2561541) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.