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Catalog Number | orb2644419 |
---|---|
Category | Antibodies |
Description | HHV8 encodes a latent nuclear antigen (LNA), which is the product of the viral gene orf 73. LNA is capable of forming a complex with retinoblastoma susceptibility gene product, which may be related to its oncogenic activity. HHV8 is associated with three different diseases observed in AIDS patients; kaposis sarcoma. |
Species/Host | Rabbit |
Clonality | Recombinant |
Clone Number | HHV8/3633R |
Tested applications | ELISA, IF, IHC-P |
Isotype | Rabbit IgG |
Immunogen | A recombinant protein corresponding to the latent nuclear antigen 1 molecule of HHV8 was used as the immunogen for the recombinant HHV8 antibody. |
Antibody Type | Primary Antibody |
Dilution range | ELISA: for coating use Ab at 1-5ug/ml (order Ab without BSA),Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT |
Purity | Protein A affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
Hazard Information | This recombinant HHV8 antibody is available for research use only. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide |
Note | For research use only |
Application notes | Optimal dilution of the recombinant HHV8 antibody should be determined by the researcher. |
Expiration Date | 12 months from date of receipt. |
IHC staining of FFPE human Karposi's sarcoma with recombinant HHV8 antibody. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant HHV8 antibody as confirmation of integrity and purity.
ELISA, IF, IHC-P | |
Rabbit | |
Recombinant | |
Unconjugated |