HHV-5 (strain AD169) gH&gL&gO, Twin-Strep Tag&Tag Free&His Tag

• Catalog Number: orb1821759
• Category: Proteins
• Description: Human cytomegalovirus (HCMV), a prototypical beta-herpes virus, is a major cause of morbidity and mortality in immunocompromised transplant recipients and congenitally infected fetuses.Different HCMV glycoprotein complexes are capable of meditating entry through interaction with cell-specific receptors on a panel of host cells. The pentamer (gH/gL/UL128/UL130/UL131A) mediates HCMV entry into epithelial, endothelial, and myeloid cells by its binding to neuropilin 2 (Nrp2). The trimer (gH/gL/gO) can mediate infection of all cell types by binding to platelet-derived growth factor-alpha (PDGFRα). Both pentamer and trimer need to interact with the glycoprotein B (gB), the fusogenic protein, to trigger the virus and host cell membrane fusion.
• Reactivity: Virus
• Tag: C-Twin Strep | Native | C-10×His
• Form/Appearance: Powder
• Purity: 90%
• Conjugation: Unconjugated
• MW: 82.6 kDa & 27.6 kDa & 52.6 kDa
• Target: gH&gL&gO
• Source: HHV-5 (strain AD169) gH&gL&gO, Twin-Strep Tag&Tag Free&His Tag is expressed from human 293 cells (HEK293). It contains AA Arg 24 - Leu 720 & Val 31 - Arg 278 & Lys 31 - Gln 466 (Accession # P12824 & P16832 & P16750).
• Expression System: HEK293
• Expression Region: Arg 24 - Leu 720 & Val 31 - Arg 278 & Lys 31 - Gln 466
• Endotoxins: 1.0 EU per μg
• Storage: -20°C
• Buffer/Preservatives: PBS, pH 7.4
• Note: For research use only
• Application notes: This protein carries a twin strep tag at the C-terminus. The protein has a calculated MW of 82.6 kDa | 27.6 kDa | 52.6 kDa. The protein migrates as 33 kDa,120-160 kDa and > 180 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.