HGD Antibody

• Catalog Number: orb412976
• Category: Antibodies
• Description: HGD Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human HGD recombinant protein (Position: D374-N445).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot,0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section),0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 5μg/ml Flow Cytometry, 1-3μg/1x106 cells Direct ELISA,0.1-0.5μg/ml
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 50 kDa
• UniProt ID: Q93099
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Homogentisate 1,2-dioxygenase; Homogentisate oxyge
• Note: For research use only
• Application notes: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of HEPG2 cells using anti-HGD antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of HGD using anti-HGD antibody.Lane 1:human HepG2 cell;2:human A549 cell;3:rat liver tissue;4:rat kidney tissue;5:mouse liver tissue;6:mouse kidney tissue.

IF analysis of HGD using anti-HGD antibody. HGD was detected in immunocytochemical section of CACO-2 cells.

IHC analysis of HGD using anti-HGD antibody.HGD was detected in paraffin-embedded section of human liver cancer tissue.

IHC analysis of HGD using anti-HGD antibody.HGD was detected in paraffin-embedded section of human prostatic cancer tissue.