HFE monoclonal antibody (M01), clone 1G12

• Catalog Number: orb2292831
• Category: Antibodies
• Description: Mouse monoclonal antibody raised against a partial recombinant HFE.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 1G12
• Tested applications: ELISA, IF, WB
• Reactivity: Human
• Isotype: IgG1 Kappa
• Immunogen: HFE (NP_000401, 115 a.a. ~ 205 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Protein Sequence: SHTLQVILGCEMQEDNSTEGYWKYGYDGQDHLEFCPDTLDWRAAEPRAWPTKLEWERHKIRARQNRAYLERDCPAQLQQLLELGRGVLDQQ
• NCBI: NP_000401
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Detection limit for recombinant GST tagged HFE is approximately 0.03 ng/ml as a capture antibody.

HFE monoclonal antibody (M01), clone 1G12. Western Blot analysis of HFE expression in A-431.

Immunofluorescence of monoclonal antibody to HFE on HeLa cell. [antibody concentration 10 ug/ml]

Western Blot analysis of HFE expression in transfected 293T cell line by HFE monoclonal antibody (M01), clone 1G12. Lane 1: HFE transfected lysate (40.1 KDa). Lane 2: Non-transfected lysate.

Western Blot detection against Immunogen (35.75 KDa).