Hfe Antibody

• Catalog Number: orb1676550
• Category: Antibodies
• Description: Hfe Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IHC, WB
• Reactivity: Mouse, Rat
• Immunogen: E.coli-derived mouse Hfe recombinant protein (Position: N112-H227).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.25-0.5 μg/ml, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Rat Flow Cytometry, 1-3 μg/1x106 cells, Mouse Direct ELISA, 0.1-0.5 μg/ml, Mouse
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 40-58 kDa
• UniProt ID: P70387
• Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.

IHC analysis of Hfe using anti-Hfe antibody. Hfe was detected in a paraffin-embedded section of rat liver tissue.

Western blot analysis of Hfe using anti-Hfe antibody.

Flow Cytometry analysis of HEPA1-6 cells using anti-Hfe antibody(Blue line).Isotype control antibody(Green line) was rabbit IgG.Unlabelled sample(Red line) was also used as a control.