HFE Antibody (Center)

• Catalog Number: orb1930001
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB19120
• Tested applications: IHC-P, WB
• Predicted Reactivity: Rat
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, IHC-P: 1:50~100
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 40108 Da
• Target: This HFE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 122-148 amino acids from the Central region of human HFE.
• UniProt ID: Q30201
• NCBI: NP_620575.1, NP_620580.1, NP_620576.1, NP_620578.1, NP_620579.1, NP_620577.1, NP_620573.1, NP_620572.1, NP_000401.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Hereditary hemochromatosis protein, HLA-H, HFE, HL
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

HFE Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HFE Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Western blot analysis of HFE antibody (Center) in Jurkat cell line lysates (35 ug/lane). HFE (arrow) was detected using the purified Pab.