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Hexokinase (HK) Activity Colorimetric Assay Kit

SKU: orb1173183

Description

Hexokinase (HK) Activity Assay Kit provides a colorimetric HK detection method. Enzyme activity is calculated by detecting the increase rate of light absorption at this wavelength.

Research Area

Signal Transduction

Images & Validation

Application Notes
Hexokinase (HK) Activity Assay Kit provides a simple, convenient, rapid and colorimetric HK detection method.HK catalyzes glucose synthesis of 6-phosphoglucose, and 6-phosphoglucose dehydrogenase further catalyzes 6-phosphoglucose dehydrogenation to NADH, which reduces WST-8 to orange formazan, with the maximum absorption peak detected at around 450 nm. The enzyme activity was calculated by detecting the increase rate of light absorption at 450 nm

Key Properties

Sample TypesSerum, Plasma, Animal and Plant Tissues, Cells, Cell Culture Supernatant, Bacteria
Range31.25-2000μM

Customer Validated Data

The data below is submitted by researchers worldwide. We provide this transparency to help you decide if this product works for your specific application or species, even if we haven't tested it ourselves.
Success by Application
Reactivity Distribution

Latest Experiments

5 Results
Dilution
Sample
Summary
Assay kit
Human
-
PCa cell lysates (22Rv1 and C4-2)
This kit was used to measure...
Assay kit
Human
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Cell lysates (HaCaT keratinocyte cell line)
This kit was used to measure...
Assay kit
Mouse
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Mouse tissue homogenates (muscle and skin) and recombinant mouse HK2 protein
This kit was used to measure...
Assay kit
Human
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HUVEC cell lysates
This kit was used to measure...
Assay kit
Monkey and Porcine
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Cell lysates (Marc-145 cells and PAMs)
This kit was used to measure...

Storage & Handling

StorageStored at -20℃ for 12 months, protected from light.
Expiration Date6 months from date of receipt.
Hazard InformationThe product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
DisclaimerFor research use only
Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Protocol Information

Available Sizes

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48 T
$ 110.00
96 T
$ 120.00
DispatchUsually dispatched within 5-10 working days
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