Cart summary

You have no items in your shopping cart.

HES1 Recombinant Rabbit Monoclonal Antibody

Catalog Number: orb1499389

DispatchUsually dispatched within 5-10 working days
$ 270.00
Catalog Numberorb1499389
CategoryAntibodies
DescriptionHES1 Recombinant Rabbit Monoclonal Antibody
Species/HostRabbit
ClonalityRecombinant
Clone Number6E11
Tested applicationsFC, ICC, IF, IHC-Fr, IHC-P, WB
Predicted ReactivityHuman, Mouse, Rat
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human HES1
Antibody TypeRecombinant Antibody
Concentration1mg/ml
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50-200, IF=1:100-500, Flow-Cyt=1ug/Test
Form/AppearanceLiquid
ConjugationUnconjugated
MW30 kDa
TargetHES1
UniProt IDQ14469
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesbHLHb39; C-HAIRY1; c-hairy1A; Class B basic helix-
Read more...
NoteFor research use only
Expiration Date12 months from date of receipt.
HES1 Recombinant Rabbit Monoclonal Antibody

ICC staining of Hes1 in 293 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (orb1499389, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1000 dilution. The nuclear counter stain is DAPI (blue).

HES1 Recombinant Rabbit Monoclonal Antibody

ICC staining of Hes1 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (orb1499389, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1000 dilution. The nuclear counter stain is DAPI (blue).

HES1 Recombinant Rabbit Monoclonal Antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-Hes1 antibody (orb1499389) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (orb1499389) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

HES1 Recombinant Rabbit Monoclonal Antibody

Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Hes1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (orb1499389, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

HES1 Recombinant Rabbit Monoclonal Antibody

Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Hes1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (orb1499389, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

HES1 Recombinant Rabbit Monoclonal Antibody

Western blot analysis of Hes1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (orb1499389, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SiHa cell lysate, Lane 2: SK-Br-3 cell lysate.