HES1 Antibody (N-term T24)

• Catalog Number: orb1930130
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB13980
• Tested applications: FC, IF, WB
• Predicted Reactivity: Mouse, Other, Rat
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: IF: 1:10~50, WB: 1:1000, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 29541 Da
• Target: This HES1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-38 amino acids from the N-terminal region of human HES1.
• UniProt ID: Q14469
• NCBI: NP_005515.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Transcription factor HES-1, Class B basic helix-lo
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

HES1 Antibody (N-term T24) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of HES1 Antibody (N-term T24) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Western blot analysis of anti-HES1 Antibody (N-term T24) in HL60 cell line lysates (35 ug/lane). HES1 (arrow) was detected using the purified Pab.