HER3 Antibody

• Catalog Number: orb1088495
• Category: Antibodies
• Description: HER3 (ERBB3) is a transmembrane protein of the epidermal growth factor receptor family, although it does not have an active tyrosine kinase domain. It can bind its ligand, but for further signaling it needs heterodimerization with other receptor tyrosine kinases of EGFR family. Overexpression of HER3 has been observed in many carcinomas. Activity of HER3 can be modulated by one of its isoforms, that is secreted from the cell, as its lacks the transmembrane domain.
• Clonality: Monoclonal
• Clone Number: H3Mab-17
• Tested applications: FC
• Reactivity: Human
• Isotype: Mouse IgG2a kappa
• Immunogen: HER3-transfected CHO cells
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 1-5 μg/ml.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: HER3
• Entrez: 2065
• UniProt ID: P21860
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-ERBB3 antibody, anti-FERLK antibody, anti-LCC
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1-5 μg/ml.
• Expiration Date: 12 months from date of receipt.

Separation of MCF-7 cells stained using anti-HER3 (H3Mab-17) purified antibody (concentration in sample 0.6 μg/ml, GAM APC, red-filled) from MCF-7 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).