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HER2/neu Antibody [SER4], Mouse IgG1

Catalog Number: orb1672026

DispatchUsually dispatched within 5-10 working days
$ 1,050.00
Catalog Numberorb1672026
CategoryAntibodies
DescriptionHER2/neu Antibody [SER4], Mouse IgG1
ClonalityRecombinant
Clone NumberSER4
Tested applicationsELISA
ReactivityHuman
IsotypeIgG1 kappa
ImmunogenRecombinant HER2/neu.
Antibody TypePrimary Antibody
Concentrationbatch dependent
ConjugationUnconjugated
TargetERBB2
UniProt IDP04626
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPBS with 0.02% Proclin 300.
Alternative namesERBB2, Receptor tyrosine-protein kinase erbB-2, hu
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NoteFor research use only
Expiration Date12 months from date of receipt.
HER2/neu Antibody [SER4], Mouse IgG1

Immunofluorescence staining of MCF7 cells using anti-HER2/neu antibody (SER4). Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells stained with the chimeric mouse IgG version of SER4 (orb1672026) at 10 ug/ml followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1672026- DAPI- merged channels and an isotype control. The isotype control was stained with anti-unknown antibody (.1) followed by Alexa Fluor® 488 secondary antibody.

HER2/neu Antibody [SER4], Mouse IgG1

Western Blot using anti-HER2/neu antibody SER4, human breast cancer tissue lysate (35 ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric mouse IgG version of SER4 (orb1672026) at 2 ug/ml before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

HER2/neu Antibody [SER4], Mouse IgG1

Flow cytometry using the anti-HER2/neu antibody SER4. Paraformaldehyde fixed MCF7 cells were stained with anti-unknown specificity antibody (.1; isotype control - black line) or the mouse IgG1 version of SER4 (orb1672026 - blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.