Hemoglobin/HBA1/HBA2 Antibody

• Catalog Number: orb18837
• Category: Antibodies
• Description: Hemoglobin/HBA1/HBA2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Hemoglobin recombinant protein (Position: V2-R142). Human Hemoglobin shares 85.8% amino acid (aa) sequence identity with mouse Hemoglobin.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, By Heat
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 15258 MW
• UniProt ID: P69905
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Hemoglobin subunit alpha;Alpha-globin;Hemoglobin a
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of K562 cells using anti-Hemoglobin antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

WB analysis of Hemoglobin using anti-Hemoglobin antibody.Lane 1:human placenta tissue.

IHC analysis of Hemoglobin using anti-Hemoglobin antibody. Hemoglobin was detected in a paraffin-embedded section of human placenta tissue.

IHC analysis of Hemoglobin using anti-Hemoglobin antibody. Hemoglobin was detected in a paraffin-embedded section of human tonsil tissue.