Helios Antibody

• Catalog Number: orb1524496
• Category: Antibodies
• Description: Helios Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human
• Immunogen: Between 25 and 75
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: Helios
• UniProt ID: Q9UKS7
• NCBI: NP_057344.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: ANF1A2; ZNFN1A2; ZNF1A2; zinc finger protein, subf
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human Helios by western blot and immunoprecipitation. Samples: Whole cell lysate (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-Helios antibody orb1524496 used for WB at 0.1 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 6 µg/mg lysate. Helios was also immunoprecipitated by rabbit anti-Helios antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).