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Catalog Number | orb234335 |
---|---|
Category | Antibodies |
Description | HEF1/NEDD9 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | WB |
Reactivity | Human, Monkey, Mouse |
Isotype | Rabbit IgG |
Immunogen | E.coli-derived human HEF1 recombinant protein (Position: K273-E421). Human HEF1 shares 83% amino acid (aa) sequence identity with mouse HEF1. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml, Human, Monkey, Mouse |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 92861 MW |
UniProt ID | Q14511 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Enhancer of filamentation 1;hEF1;CRK-associated su Read more... |
Note | For research use only |
Application notes | WB: The detection limit for HEF1 is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis using Anti-HEF1 Picoband antibody.Lane 1:JURKAT Cell;2:CEM Cell;3:RAJI Cell.
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