HECTD3 (vPairTM) Antibodies

• Catalog Number: orb131755
• Category: Antibodies
• Description: Rabbit polyclonal to HECTD3. HECTD3 antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Biorbyt to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody pairs-based assays. The use of a pair of fully characterized vPair antibodies in the same assay can validate signal specificity since vPair antibodies recognize two independent epitopes of the same protein. Different sets of vPair antibodies are developed at Biorbyt to work with specific applications, including antibody pairs arrays, Western blot, IP-Western, ChIP, IHC, and FACS. E3 ubiquitin-protein ligase HECTD3 accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. HECTD3 mediates ubiquitination of TRIOBP and its subsequent proteasomal degradation, thus faciliting cell cycle progression by regulating the turn-over of TRIOBP.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human, Mouse
• Immunogen: N-terminal or the C-terminal region of human HECTD3
• Concentration: 100 μg/ml BSA
• Dilution range: WB:1:1000-1:3000
• Purity: Affinity purification
• Conjugation: Unconjugated
• MW: 97
• UniProt ID: Q5T447
• Storage: -20°C
• Buffer/Preservatives: 0.01% NaN3
• Alternative names: anti E3 ubiquitin-protein ligase HECTD3 antibody p
• Note: For research use only
• Application notes: < strong>WB: The apparent protein size on WB may be different from the calculated M.W. due to modifications.; < br>< strong>Experiment Notes: Rabbit polyclonal antibodies were produced by immunizing animals with GST-fusion proteins containing either the N-terminal or the C-terminal region of human HECTD3.
• Expiration Date: 12 months from date of receipt.