HE4/Wfdc2 Antibody

• Catalog Number: orb402508
• Category: Antibodies
• Description: HE4/Wfdc2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived rat HE4 recombinant protein (Position: E31-F168).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml ELISA (Cap), 1-5μg/ml
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 19 kDa
• UniProt ID: Q8CHN3
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: WAP four-disulfide core domain protein 2; Epididym
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of HE4 using anti-HE4 antibody.Lane 1:rat testis tissue;2:rat brain tissue;3:rat thymus tissue;4:mouse testis tissue;5:mouse brain tissue;6:mouse thymus tissue;7:mouse HEPA1-6 cell.

Sandwich ELISA - Recombinant rat HE4/Wfdc2 protein standard curve.Use in combination with reagents from Rat HE4/Wfdc2 ELISA Kit EZ-Set (DIY Antibody Pairs).

IHC analysis of HE4 using anti-HE4 antibody.HE4 was detected in paraffin-embedded section of mouse lung tissue.

IHC analysis of HE4 using anti-HE4 antibody.HE4 was detected in paraffin-embedded section of mouse small intestine tissue.

IHC analysis of HE4 using anti-HE4 antibody.HE4 was detected in paraffin-embedded section of rat kidney tissue.

IHC analysis of HE4 using anti-HE4 antibody.HE4 was detected in paraffin-embedded section of rat lung tissue.

IHC analysis of HE4 using anti-HE4 antibody.HE4 was detected in paraffin-embedded section of rat small intestine tissue.