HDGF Antibody

• Catalog Number: orb1525327
• Category: Antibodies
• Description: HDGF Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: between 200 and 240
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: HDGF
• UniProt ID: P51858
• NCBI: NP_004485.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: high mobility group protein 1-like 2; epididymis s
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human and mouse HDGF by western blot (h and m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , Jurkat (J; 50 µg) , HEK293T (T; 50 µg) , and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-HDGF antibody orb1525327 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. HDGF was also immunoprecipitated by rabbit anti-HDGF antibodies, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).

Detection of mouse HDGF by immunohistochemistry. Samples: FFPE serial sections of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-HDGF used at a dilution of 1: 1, 000 (1µg/ml) or 1: 200 (1µg/ml). Detection: DAB

Detection of human HDGF by immunohistochemistry. Samples: FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti-HDGF used at a dilution of 1: 1, 000 (1µg/ml) or 1: 200 (1µg/ml). Detection: DAB. Counterstain: Hematoxylin (blue).