HDAC7A monoclonal antibody (M01), clone 2B11-1C1

• Catalog Number: orb2291126
• Category: Antibodies
• Description: Mouse monoclonal antibody raised against a full length recombinant HDAC7A.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG2b Kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Immunogen: HDAC7A (AAH06453.1, 1 a.a. ~ 276 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Protein Sequence: MGFCFFNSVAIACRQLQQQSKASKILIVDWDVHHGNGTQQTFYQDPSVLYISLHRHDDGNFFPGSGAVDEVGAGSGEGFNVNVAWAGGLDPPMGDPEYLAAFRIVVMPIAREFSPDLVLVSAGFDAAEGHPAPLGGYHVSAKCFGYMTQQLMNLAGGAVVLALEGGHDLTAICDASEACVAALLGNRVDPLSEEGWKQKPNLNAIRSLEAVIRVHSKYWGCMQRLASCPDSWVPRVPGADKEEVEAVTALASLSVGILAEDRPSEQLVEEEEPMNL
• Tested applications: ELISA, WB
• Clone Number: 2B11-1C1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• NCBI: AAH06453.1

Detection limit for recombinant GST tagged HDAC7 is 0.3 ng/ml as a capture antibody.

HDAC7A monoclonal antibody (M01), clone 2B11-1C1 Western Blot analysis of HDAC7A expression in K-562.

Western Blot analysis of HDAC7A expression in transfected 293T cell line by HDAC7A monoclonal antibody (M01), clone 2B11-1C1. Lane 1: HDAC7A transfected lysate(29.6 KDa). Lane 2: Non-transfected lysate.

Western blot analysis of HDAC7A over-expressed 293 cell line, cotransfected with HDAC7A Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with HDAC7A monoclonal antibody (M01), clone 2B11-1C1 (Cat # orb2291126). GAPDH (36.1 kDa) used as specificity and loading control.

Western Blot detection against Immunogen (56.1 KDa).