HDAC7 (Phospho-S155) antibody

• Catalog Number: orb393312
• Category: Antibodies
• Description: Rabbit polyclonal antibody to HDAC7 (Phospho-S155)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat, Zebrafish
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S155 of human Histone Deacetylase 7 protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500:1000, IF/ICC: 1:100:500
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: HDAC7
• Entrez: 51564, 56233
• UniProt ID: Q8WUI4, Q8C2B3, Q99P96
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: HDAC7A; Histone deacetylase 7; HD7; Histone deacet
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Histone Deacetylase 7 (Phospho-S155) expression in HEK293T (A), Hela (B), U2OS (C), rat lung (D) whole cell lysates. (Predicted band size: 102 kD; Observed band size: 105 kD)

Immunofluorescent analysis of Histone Deacetylase 7 (Phospho-S155) staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.